Anticorps Monoclonal anti-SLC39A3

SLC39A3 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1A11F6

N° de cat : 68025-1-Ig

Synonymes

SLC39A3, Zinc transporter ZIP3, ZIP 3, ZIP3, Zrt and Irt like protein 3

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Galerie de données de validation

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  WB analysis using 68025-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of HepG2 using 68025-1-Ig

  unboiled HepG2 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68025-1-Ig

  37°C incubated A549 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 68025-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 68025-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 68025-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 68025-1-Ig (SLC39A3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HepG2 non bouillies, cellules A549, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68025-1-Ig cible SLC39A3 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC39A3 Protéine recombinante Ag14289
• Nom complet: solute carrier family 39 (zinc transporter), member 3
• Masse moléculaire calculée: 314 aa, 34 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC005869
• Symbole du gène: SLC39A3
• Identification du gène (NCBI): 29985
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC39A3, also named as ZIP3, belongs to the ZIP transporter (TC 2.A.5) family. SLC39A3 is a Zn importer that acts as a zinc-influx transporter. SLC39A3 is highly expressed in a cell-type-specific manner in ovaries, testes, and the developing nervous system with limited expression in the small intestinal crypt cells, kidney glomerulus, and discrete regions of the liver. SLC39A3 is also expressed in highly specialized, hormonally responsive secretory tissues, such as the pancreas, prostate, and mammary gland (PMID: 19458277). SLC39A3 has 2 isoforms with the molecular mass of 11 and 34 kDa.