Anticorps Polyclonal de lapin anti-ZIP8

• Phare
• Validé par KD/KO

ZIP8 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

34

N° de cat : 20459-1-AP

Synonymes

BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8

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Galerie de données de validation

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  WB analysis of mouse lung using 20459-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 20459-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 20459-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 20459-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20459-1-AP (ZIP8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 20459-1-AP

  IP Result of anti-ZIP8 (IP:20459-1-AP, 4ug; Detection:20459-1-AP 1:500) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human kidney using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20459-1-AP (ZIP8 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20459-1-AP (ZIP8 antibody) at dilution of 1:800 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20459-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 20459-1-AP (ZIP8 antibody) at dilution of 1:100 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, cellules U-937, tissu cardiaque de souris, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'intestin grêle de souris, tissu placentaire humain, tissu pulmonaire de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 25 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Informations sur le produit

20459-1-AP cible ZIP8 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ZIP8 Protéine recombinante Ag14292
• Nom complet: solute carrier family 39 (zinc transporter), member 8
• Masse moléculaire calculée: 460 aa, 50 kDa
• Poids moléculaire observé: 42-46 kDa, 75-90 kDa
• Numéro d’acquisition GenBank: BC012125
• Symbole du gène: SLC39A8
• Identification du gène (NCBI): 64116
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.

Publications

Species	Application	Title
human	IHC	Cell; Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis.; Authors - Jin-Hong Kim
mouse	WB,IF	J Clin Invest; Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.; Authors - Wen Lin ; KO Validated
mouse	WB	Acta Neuropathol Commun; Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.; Authors - Alexandra Grubman
rat	WB	Int J Mol Sci; Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes.; Authors - Yusuf Olgar
mouse	WB,IF	Int Immunopharmacol; ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.; Authors - Gong Cheng ; KD Validated
human	IHC	Front Cell Dev Biol; An Iron Metabolism-Related Gene Signature for the Prognosis of Colon Cancer.; Authors - Jing Yuan ; KD Validated