Anticorps Polyclonal de lapin anti-SLC3A1

• Phare
• Validé par KD/KO

SLC3A1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 16343-1-AP

Synonymes

ATR1, CSNU1, D2H, NBAT, RBAT, SLC3A1

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Galerie de données de validation

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  WB analysis using 16343-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16343-1-AP (SLC3A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 16343-1-AP

  IP Result of anti-SLC3A1 (IP:16343-1-AP, 4ug; Detection:16343-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16343-1-AP (SLC3A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16343-1-AP (SLC3A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16343-1-AP (SLC3A1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human small intestine using 16343-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16343-1-AP (SLC3A1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu rénal humain, tissu d'intestin grêle humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

16343-1-AP cible SLC3A1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC3A1 Protéine recombinante Ag9525
• Nom complet: solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
• Masse moléculaire calculée: 685 aa, 79 kDa
• Poids moléculaire observé: 79 kDa
• Numéro d’acquisition GenBank: BC022386
• Symbole du gène: SLC3A1
• Identification du gène (NCBI): 6519
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC3A1(Neutral and basic amino acid transport protein rBAT) is also named as NBAT,D2h,RBAT. It is involved in the high-affinity, sodium-independent transport of cystine and neutral and dibasic amino acids (system B(0,+)-like activity).SLC3A1 can be degraded, at least in part, via the endoplasmic reticulum-associated degradation (ERAD) pathway(PMID:18332091). SLC3A1 has different isoforms vary from 79 kDa to 36kDa and the 85 kDa glycosylated protein can be detected in SDS-PAGE. (PMID:11546643)

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney.; Authors - M Takasato
mouse	WB	Nat Commun; Transcription factors AP-2α and AP-2β regulate distinct segments of the distal nephron in the mammalian kidney.; Authors - Joseph O Lamontagne
mouse	IF	Dev Cell; AP-2β/KCTD1 Control Distal Nephron Differentiation and Protect against Renal Fibrosis.; Authors - Alexander G Marneros
human	WB	Theranostics; Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.; Authors - Yang Jiang ; KD Validated
mouse	IF	Sci Rep; Kidney-specific transposon-mediated gene transfer in vivo.; Authors - Lauren E Woodard
mouse	WB	FASEB J; Epidermal growth factor deficiency predisposes to progressive renal disease.; Authors - Alia M Zeid

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Felisha (Verified Customer) (01-03-2020)

The primary antibody worked great to localize the derived human stem cells in the mouse tissue samples that were sectioned and paraffin embedded. Wonderful for immunohistochemistry!

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1: 200
• Cell Tissue Type: Mouse tissue and human cells