Anticorps Polyclonal de lapin anti-CD98/SLC3A2

• Phare
• Validé par KD/KO

CD98/SLC3A2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

30

N° de cat : 15193-1-AP

Synonymes

4F2, 4F2 heavy chain antigen, 4F2HC, 4T2HC, CD98, CD98/SLC3A2, CD98HC, MDU1, NACAE, SLC3A2

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Galerie de données de validation

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  WB analysis of HeLa using 15193-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 15193-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15193-1-AP (CD98 antibody at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15193-1-AP (CD98/SLC3A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15193-1-AP (CD98 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of A549 using 15193-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD98/SLC3A2 antibody (15193-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 15193-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human CD98/SLC3A2 (15193-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Raji
• Résultats positifs en IHC: tissu testiculaire de rat, tissu placentaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2, cellules A549
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

15193-1-AP cible CD98/SLC3A2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD98/SLC3A2 Protéine recombinante Ag7280
• Nom complet: solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 85-94 kDa, 120-130 kDa
• Numéro d’acquisition GenBank: BC001061
• Symbole du gène: SLC3A2
• Identification du gène (NCBI): 6520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD98 is a cell-surface heterodimer consisting of a heavy chain (CD98hc) and a light chain. CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). This protein has 4 isoforms with the molecular mass of 58-71 kDa and can be detected 85-94 kDa due to glycosylation. The glycosylated CD98hc can links to a non-glycosylated light chain (~40 kDa) via a disulfide bond to form a heterodimeric CD98 antigen with molecular mass of 120-130 kDa (PMID: 14770309).

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering MMP-2 Activated Nanoparticles Carrying B7-H3 Bispecific Antibodies for Ferroptosis-Enhanced Glioblastoma Immunotherapy; Authors - Rangrang Fan
human	WB,IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
mouse	IF	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	IF	J Allergy Clin Immunol; Mast cell surfaceome characterization reveals CD98hc is critical for optimal cell function.; Authors - Siddhartha S Saha
human	WB	J Hazard Mater; ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes.; Authors - Xuan Lai
mouse	WB	Cell Rep; Brown adipocyte ATF4 activation improves thermoregulation and systemic metabolism; Authors - Esther Paulo