Anticorps Polyclonal de lapin anti-SLC43A2

SLC43A2 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30031-1-PBS

Synonymes

LAT4, L-type amino acid transporter 4, PP7664

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Galerie de données de validation

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  WB analysis using 30031-1-AP (same clone as 30031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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  WB analysis using 30031-1-AP (same clone as 30031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30031-1-AP (same clone as 30031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 30031-1-AP (same clone as 30031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30031-1-AP (SLC43A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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  IF Staining of human lung cancer using 30031-1-AP (same clone as 30031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using SLC43A2 antibody (30031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 30031-1-AP (same clone as 30031-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SLC43A2 Polyclonal antibody (30031-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 30031-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30031-1-PBS cible SLC43A2 dans les applications de WB, IHC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC43A2 Protéine recombinante Ag31341
• Nom complet: solute carrier family 43, member 2
• Masse moléculaire calculée: 63 kDa
• Poids moléculaire observé: 48-53 kDa
• Numéro d’acquisition GenBank: BC027923
• Symbole du gène: SLC43A2
• Identification du gène (NCBI): 124935
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC43A2 (solute carrier family 43 member 2, also known as LAT4) is a multi-pass transmembrane protein that mediates sodium- and chloride-independent uptake of large neutral amino acids, including methionine, leucine and phenylalanine. Highly expressed in kidney, placenta and liver, it supplies essential amino acids to support fetal growth, protein synthesis and one-carbon metabolism. Over-expressed in head-and-neck, liver and other cancers, SLC43A2-driven methionine influx maintains methylation reactions and YAP activity, promoting tumor proliferation and chemo-resistance . Pharmacological inhibition of SLC43A2 sensitizes tumors to therapy, highlighting its potential as a metabolic target. (PMID: 39747898; 15659399; 30379325)

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