Anticorps Polyclonal de lapin anti-SLC6A8

• Phare
• Validé par KD/KO

SLC6A8 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 20299-1-AP

Synonymes

Creatine transporter 1, CRT, CRTR, CT1, SLC6A8

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Galerie de données de validation

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  WB analysis using 20299-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 20299-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 20299-1-AP (SLC6A8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20299-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20299-1-AP (SLC6A8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 20299-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 20299-1-AP (SLC6A8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of PC-3 using 20299-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using 20299-1-AP (SLC6A8 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HUVEC, tissu cardiaque de rat, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 5 publications below

Informations sur le produit

20299-1-AP cible SLC6A8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC6A8 Protéine recombinante Ag14110
• Nom complet: solute carrier family 6 (neurotransmitter transporter, creatine), member 8
• Masse moléculaire calculée: 635 aa, 71 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC012355
• Symbole du gène: SLC6A8
• Identification du gène (NCBI): 6535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC6A8, also known as the sodium- and chloride-dependent creatine transporter 1 (CT1), plays a critical role in transporting creatine, a crucial molecule for energy metabolism, into cells. SLC6A8 belongs to the solute carrier family 6 (SLC6), responsible for transporting diverse molecules across cell membranes. SLC6A8 expression is highest in muscle, kidney, and other tissues with high energy demands. Mutations in SLC6A8 cause creatine transporter deficiency, an X-linked mental retardation disorder (PMID: 17465020).

Publications

Species	Application	Title
human	IHC	Front Oncol; Long Non-Coding RNA AL513318.2 as ceRNA Binding to hsa-miR-26a-5p Upregulates SLC6A8 Expression and Predicts Poor Prognosis in Non-Small Lung Cancer.; Authors - Yongfei Fan
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa
human	WB,IHC	Ann Transl Med; SLC6A8 is involved in the progression of non-small cell lung cancer through the Notch signaling pathway.; Authors - Yan Feng ; KD Validated
human	IHC	Front Genet; SLC6A8 is a Potential Biomarker for Poor Prognosis in Lung Adenocarcinoma.; Authors - Yongfei Fan
human	WB	Technol Cancer Res Treat; SLC6A8 Knockdown Suppresses the Invasion and Migration of Human Hepatocellular Carcinoma Huh-7 and Hep3B Cells.; Authors - Lu Yuan ; KD Validated
mouse	WB	Oncoimmunology; CARD9 deficiency promotes pancreatic cancer growth by blocking dendritic cell maturation via SLC6A8-mediated creatine transport; Authors - Cheng Tian