Anticorps Polyclonal de lapin anti-SLC7A5

• Phare
• Validé par KD/KO

SLC7A5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(15)

Avis (1)

N° de cat : 28670-1-AP

Synonymes

LAT1, 4F2 LC, 4F2 light chain, 4F2LC, CD98 light chain

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Galerie de données de validation

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  WB analysis using 28670-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28670-1-AP (SLC7A5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 28670-1-AP

  WB result of SLC7A5 antibody (28670-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC7A5 transfected MCF-7 cells.

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  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28670-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28670-1-AP (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 28670-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HT-29 cells using SLC7A5 antibody (28670-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, cellules MKN-45, cellules SW480
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 3 publications below

Informations sur le produit

28670-1-AP cible SLC7A5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag29164
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Publications

Species	Application	Title
human	WB,IHC	Eur J Nucl Med Mol Imaging; Synthesis, preclinical assessment, and first-in-human study of [18F]d4-FET for brain tumor imaging; Authors - Lu Hou
human	WB	Mol Metab; RNA-binding protein YBX3 promotes PPARγ-SLC3A2 mediated BCAA metabolism fueling brown adipogenesis and thermogenesis; Authors - Lin-Yun Chen
	WB	J Inherit Metab Dis; Metabolic characterization of neurogenetic disorders involving glutamatergic neurotransmission; Authors - Sofía Illescas
human	WB	Oncol Lett; SLC7A5 regulates tryptophan uptake and PD‑L1 expression levels via the kynurenine pathway in ovarian cancer; Authors - Ruibin Jiang
human	WB	J Exp Clin Cancer Res; CircARID1A binds to IGF2BP3 in gastric cancer and promotes cancer proliferation by forming a circARID1A-IGF2BP3-SLC7A5 RNA-protein ternary complex; Authors - Qiang Ma
human	WB,IHC	Front Oncol; ZNF24 regulates the progression of KRAS mutant lung adenocarcinoma by promoting SLC7A5 translation; Authors - Daqi Jia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xie (Verified Customer) (07-07-2023)

The expression of Slc7a5 is good for my samples

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: brain

SLC7A5 Antibody Immunofluorescence validation (1:250 dilution) in brain (Cat no:28670-1-AP)

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