Anticorps Monoclonal anti-SLC7A5

SLC7A5 Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2G5H3

N° de cat : 67951-1-Ig

Synonymes

LAT1, 2G5H3, 4F2 LC, 4F2 light chain, 4F2LC

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Galerie de données de validation

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  WB analysis using 67951-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67951-1-Ig (SLC7A5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

67951-1-Ig cible SLC7A5 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag20291
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 35-50 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

Publications

Species	Application	Title
human	WB	Nat Metab; Extracellular acidosis restricts one-carbon metabolism and preserves T cell stemness; Authors - Hongcheng Cheng
		J Med Chem; Discovery of Novel Aminobutanoic Acid-Based ASCT2 Inhibitors for the Treatment of Non-Small-Cell Lung Cancer; Authors - Lian Qin
goat	IF	Anim Nutr; Long-term leucine supplementation increases body weight in goats by controlling appetite and muscle protein synthesis under protein-restricted conditions; Authors - Xiaokang Lv

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