Anticorps Recombinant de lapin anti-SLC7A5

SLC7A5 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241476D5

N° de cat : 84178-5-PBS

Synonymes

LAT1, 241476D5, 4F2 LC, 4F2 light chain, 4F2LC

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Galerie de données de validation

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  WB analysis of A549 using 84178-5-RR (same clone as 84178-5-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 84178-5-RR (SLC7A5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84178-5-RR (same clone as 84178-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

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  IF Staining of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using SLC7A5 antibody (84178-5-RR, Clone: 241476D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

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  FC experiment of HT-29 using 84178-5-RR (same clone as 84178-5-PBS)

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug SLC7A5 Recombinant antibody (84178-5-RR, Clone:241476D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84178-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84178-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84178-5-RR against Human SLC7A5 were performed. The affinity constant is 0.319 nM.

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Informations sur le produit

84178-5-PBS cible SLC7A5 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag29164
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

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