Anticorps Recombinant de lapin anti-SLC7A5

SLC7A5 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241476D5

N° de cat : 84178-5-RR

Synonymes

LAT1, 241476D5, 4F2 LC, 4F2 light chain, 4F2LC

---

Galerie de données de validation

• 

  WB analysis of A549 using 84178-5-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 84178-5-RR (SLC7A5 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 84178-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84178-5-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84178-5-RR (SLC7A5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 84178-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HT-29 cells using SLC7A5 antibody (84178-5-RR, Clone: 241476D5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of HT-29 using 84178-5-RR

  1x10^6 HT-29 cells were intracellularly stained with 0.25 ug SLC7A5 Recombinant antibody (84178-5-RR, Clone:241476D5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 84178-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84178-5-RR against Human SLC7A5 were performed. The affinity constant is 0.319 nM.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules MCF-7, cellules MKN-45, cellules SW480
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HT-29,
• Résultats positifs en FC (Intra): cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84178-5-RR cible SLC7A5 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SLC7A5 Protéine recombinante Ag29164
• Nom complet: solute carrier family 7 (cationic amino acid transporter, y+ system), member 5
• Masse moléculaire calculée: 507 aa, 55 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC039692
• Symbole du gène: SLC7A5
• Identification du gène (NCBI): 8140
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Large neutral amino acid transporter 1 LAT1(also named as SLC7A5) is a sodium- and pH-independent transporter that supplies essential amino acids (e.g., leucine, phenylalanine) to cells. It plays an important role in the blood-brain barrier (BBB), where it facilitates the transport of thyroid hormones, drugs (e.g., l-DOPA, gabapentin), and metabolites to the brain. In addition, its expression is highly upregulated in various types of human cancers, which are characterized by a high demand for amino acids for growth and proliferation. The LAT1 subunit in humans is a 507 amino acid-long polypeptide with a theoretical molecular mass of 55 kDa. The protein is hydrophobic and is predicted to be constituted by 12 transmembrane segments. The apparent molecular mass of the proteins diminished to approximately 35 - 40 kDa.(PMID: 23912240 ;PMID: 26256001)

{{ptg:RelatedPrimaryAntibodies}}