Anticorps Polyclonal de lapin anti-SLC9A9

SLC9A9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13718-1-AP

Synonymes

NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9

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Galerie de données de validation

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  WB analysis of Raji using 13718-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13718-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13718-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13718-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13718-1-AP (SLC9A9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:600
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

13718-1-AP cible SLC9A9 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC9A9 Protéine recombinante Ag4660
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 9
• Masse moléculaire calculée: 645 aa, 73 kDa
• Poids moléculaire observé: 66-73 kDa
• Numéro d’acquisition GenBank: BC035779
• Symbole du gène: SLC9A9
• Identification du gène (NCBI): 285195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Cell Commun Signal; MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells.; Authors - Daniela M Gomez Zubieta
human	WB	Am J Med Genet B Neuropsychiatr Genet; Autism spectrum disorder traits in Slc9a9 knock-out mice.; Authors - Lina Yang
human	WB, CoIP	Atten Defic Hyperact Disord; Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism.; Authors - Yanli Zhang-James
human	WB	Mol Cancer Res; An Endosomal Acid-Regulatory Feedback System Rewires Cytosolic cAMP Metabolism and Drives Tumor Progression; Authors - Hari Prasad

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