Anticorps Monoclonal anti-SLC9A9

SLC9A9 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF,ELISA

Conjugaison

Non conjugué

CloneNo.

1B8D9

N° de cat : 66577-1-Ig

Synonymes

Na(+)/H(+) exchanger 9, Nbla00118, NHE 9, NHE9, SLC9A9, Sodium/hydrogen exchanger 9

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Galerie de données de validation

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  WB analysis using 66577-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66577-1-Ig (SLC9A9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66577-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66577-1-Ig (SLC9A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66577-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLC9A9 antibody (66577-1-Ig, Clone: 1B8D9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules MCF-7, cellules NIH/3T3, cellules RAW 264.7
• Résultats positifs en IHC: tissu rénal humain, tissu d'amygdalite humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66577-1-Ig cible SLC9A9 dans les applications de WB, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SLC9A9 Protéine recombinante Ag4660
• Nom complet: solute carrier family 9 (sodium/hydrogen exchanger), member 9
• Masse moléculaire calculée: 645 aa, 73 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC035779
• Symbole du gène: SLC9A9
• Identification du gène (NCBI): 285195
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.