Anticorps Polyclonal de lapin anti-PGT

PGT Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 14327-1-AP

Synonymes

SLCO2A1, PHOAR2, OATP2A1

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Galerie de données de validation

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  WB analysis using 14327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14327-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14327-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14327-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14327-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 14327-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14327-1-AP (PGT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules NIH/3T3, tissu hépatique de souris, tissu rénal de rat
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 2 publications below

Informations sur le produit

14327-1-AP cible PGT dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGT Protéine recombinante Ag5594
• Nom complet: solute carrier organic anion transporter family, member 2A1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 65 kDa
• Numéro d’acquisition GenBank: BC041140
• Symbole du gène: PGT
• Identification du gène (NCBI): 6578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (8787677). This antibody is expected to detect the endogenous PGT protein.

Publications

Species	Application	Title
pig	IHC	Biol Reprod; Prostaglandin Transporters ABCC4 and SLCO2A1 in the Uterine Endometrium and Conceptus During Pregnancy in Pigs.; Authors - Heewon Seo
mouse	WB	Int Immunopharmacol; Effect of oridonin on oxylipins in the livers of mice with acute liver injury induced by D-galactosamine and lipopolysaccharide.; Authors - Zhikun Zhan
human	IHC	Dig Dis Sci; Four Variants of SLCO2A1 Identified in Three Chinese Patients with Chronic Enteropathy Associated with the SLCO2A1 Gene.; Authors - Hui Huang

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