Anticorps Polyclonal de lapin anti-SLIRP

• Phare
• Validé par KD/KO

SLIRP Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26006-1-PBS

Synonymes

C14orf156, DC23, DC50, PD04872, SRA stem-loop-interacting RNA-binding protein, mitochondrial

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Galerie de données de validation

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  WB analysis using 26006-1-AP (same clone as 26006-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 26006-1-AP (same clone as 26006-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 26006-1-AP (same clone as 26006-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26006-1-AP (SLIRP antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 26006-1-AP (same clone as 26006-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26006-1-AP (SLIRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 26006-1-AP (same clone as 26006-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SLIRP antibody (26006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26006-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26006-1-PBS cible SLIRP dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLIRP Protéine recombinante Ag22945
• Nom complet: chromosome 14 open reading frame 156
• Masse moléculaire calculée: 109 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC017895
• Symbole du gène: SLIRP
• Identification du gène (NCBI): 81892
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLIRP, also named as C14orf156, DC23, DC50 and PD04872. It is an RNA-binding protein on steroid receptor RNA activator (SRA), and its expression is ubiquitous in human normal tissues. SLIRP can affect mitochondrial mRNA transcription and energy conversion. In addition, the loss or weakening of SLIRP destroys part of the structure of mitochondria, affects mitochondria energy metabolism, and increases sperm or cell oxidative stress damage (PMID: 33150185). SLIRP has 2 isoforms with the molecular mass of 12 kDa.

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