Anticorps Recombinant de lapin anti-SLIT2-Specific

SLIT2-Specific Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250648A6

N° de cat : 82725-4-PBS

Synonymes

SLIT2, SLIL3, Slit 2, Slit homolog 2 protein, Slit homolog 2 protein C-product

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Galerie de données de validation

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  WB analysis of HEK-293 using 82725-4-RR (same clone as 82725-4-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 82725-4-RR (same clone as 82725-4-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 82725-4-RR (same clone as 82725-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 82725-4-RR (same clone as 82725-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82725-4-PBS in a different storage buffer formulation.

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Informations sur le produit

82725-4-PBS cible SLIT2-Specific dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: slit homolog 2 (Drosophila)
• Masse moléculaire calculée: 170 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_004787
• Symbole du gène: SLIT2
• Identification du gène (NCBI): 9353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.

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