Anticorps Recombinant de lapin anti-SLIT2-Specific

SLIT2-Specific Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

250648A6

N° de cat : 82725-4-RR

Synonymes

SLIT2, SLIL3, Slit 2, Slit homolog 2 protein, Slit homolog 2 protein C-product

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Galerie de données de validation

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  WB analysis of HEK-293 using 82725-4-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 82725-4-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 82725-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 82725-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82725-4-RR (SLIT2-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu pulmonaire de souris
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82725-4-RR cible SLIT2-Specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: slit homolog 2 (Drosophila)
• Masse moléculaire calculée: 170 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_004787
• Symbole du gène: SLIT2
• Identification du gène (NCBI): 9353
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLIT2, also named as SLIL3, is thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development it is involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development, SLIT2 may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. SLIT2 may be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system it appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, it collapses and repels retinal ganglion cell growth cones. SLIT2 seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. It seems to be involved in regulating leukocyte migration. The antibody is specific to SLIT2.

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