Anticorps Polyclonal de lapin anti-SLK

SLK Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19743-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19743-1-AP (same clone as 19743-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 19743-1-AP (same clone as 19743-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 19743-1-AP (same clone as 19743-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 19743-1-AP (same clone as 19743-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19743-1-AP (SLK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 19743-1-AP (same clone as 19743-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLK antibody (19743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 19743-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19743-1-PBS cible SLK dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: STE20-like kinase (yeast)
• Masse moléculaire calculée: 143 kDa
• Poids moléculaire observé: 143-160 kDa, 210-220 kDa
• Numéro d’acquisition GenBank: NM_014720
• Symbole du gène: SLK
• Identification du gène (NCBI): 9748
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)

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