Anticorps Polyclonal de lapin anti-SLPI

SLPI Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 32205-1-AP

Synonymes

ALK1, ALP, Antileukoprotease, Antileukoproteinase, HUSI-1

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Galerie de données de validation

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  WB analysis using 32205-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32205-1-AP (SLPI antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug.

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  IP experiment of HeLa using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug.

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  IP experiment of human saliva using 32205-1-AP

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:1000) with human saliva lysate 400 ug.

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  IF Staining of HeLa using 32205-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLPI antibody (32205-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: cellules HeLa, salive humain, salive humaine
• Résultats positifs en IP: cellules HeLa, salive humain, salive humaine
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

32205-1-AP cible SLPI dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLPI Protéine recombinante Ag36523
• Nom complet: secretory leukocyte peptidase inhibitor
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC020708
• Symbole du gène: SLPI
• Identification du gène (NCBI): 6590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Secretory leukocyte protease inhibitor (SLPI) is also named as Antileukoproteinase (ALP), BLPI, HUSI-1, WAP4, WFDC4 and mucus proteinase inhibitor (MPI). The SLPI is a multifunctional protein involved in the modulation of immunological response and the inhibition of protease activities. SLPI acts as an inhibitor of proteases, exerts antibacterial properties, and suppresses the transcription of proinflammatory genes through the nuclear factor-kappa B (NF-κB) pathway (PMID: 37356220). SLPI is as a serine protease inhibitor and also as a critical mediator that is involved in the PTH-mediated shift to the osteoblastic phase (PMID: 17474882). Slpi induction in osteoblasts enhances its differentiation, and increases osteoblast-osteoclast contact, thereby suppressing osteoclastic function (PMID: 17474882). SLPI is an evolutionarily conserved, pleiotropic protein expressed at mucosal surfaces, mainly by epithelial cells (PMID: 33602652). SLPI maintains homeostasis at barrier tissues by preventing tissue destruction and regulating the threshold of inflammatory immune responses, while protecting the host from infection. However, as recent studies demonstrate that overexpression of SLPI increases the metastatic potential of epithelial tumors (PMID: 33602652). The role of this protein as a regulatory agent has been implicated in various types of cancer (PMID: 37356220).

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