Anticorps Polyclonal de lapin anti-SLPI

SLPI Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32205-1-PBS

Synonymes

ALK1, ALP, Antileukoprotease, Antileukoproteinase, HUSI-1

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Galerie de données de validation

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  WB analysis using 32205-1-AP (same clone as 32205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32205-1-AP (SLPI antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32205-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 32205-1-AP (same clone as 32205-1-PBS)

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 32205-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 32205-1-AP (same clone as 32205-1-PBS)

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:800) with HeLa cells lysate 1200 ug. This data was developed using the same antibody clone with 32205-1-PBS in a different storage buffer formulation.

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  IP experiment of human saliva using 32205-1-AP (same clone as 32205-1-PBS)

  IP result of anti-SLPI (IP:32205-1-AP, 4ug; Detection:32205-1-AP 1:1000) with human saliva lysate 400 ug. This data was developed using the same antibody clone with 32205-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 32205-1-AP (same clone as 32205-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SLPI antibody (32205-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32205-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32205-1-PBS cible SLPI dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLPI Protéine recombinante Ag36523
• Nom complet: secretory leukocyte peptidase inhibitor
• Masse moléculaire calculée: 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC020708
• Symbole du gène: SLPI
• Identification du gène (NCBI): 6590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Secretory leukocyte protease inhibitor (SLPI) is also named as Antileukoproteinase (ALP), BLPI, HUSI-1, WAP4, WFDC4 and mucus proteinase inhibitor (MPI). The SLPI is a multifunctional protein involved in the modulation of immunological response and the inhibition of protease activities. SLPI acts as an inhibitor of proteases, exerts antibacterial properties, and suppresses the transcription of proinflammatory genes through the nuclear factor-kappa B (NF-κB) pathway (PMID: 37356220). SLPI is as a serine protease inhibitor and also as a critical mediator that is involved in the PTH-mediated shift to the osteoblastic phase (PMID: 17474882). Slpi induction in osteoblasts enhances its differentiation, and increases osteoblast-osteoclast contact, thereby suppressing osteoclastic function (PMID: 17474882). SLPI is an evolutionarily conserved, pleiotropic protein expressed at mucosal surfaces, mainly by epithelial cells (PMID: 33602652). SLPI maintains homeostasis at barrier tissues by preventing tissue destruction and regulating the threshold of inflammatory immune responses, while protecting the host from infection. However, as recent studies demonstrate that overexpression of SLPI increases the metastatic potential of epithelial tumors (PMID: 33602652). The role of this protein as a regulatory agent has been implicated in various types of cancer (PMID: 37356220).

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