Anticorps Monoclonal anti-SMAD1

SMAD1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D10B8

N° de cat : 66559-1-PBS

Synonymes

2D10B8, BSP1, BSP-1, hSMAD1, JV41

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Galerie de données de validation

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  WB analysis using 66559-1-Ig (same clone as 66559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66559-1-Ig (same clone as 66559-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66559-1-Ig (SMAD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66559-1-Ig (same clone as 66559-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66559-1-Ig (SMAD1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66559-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66559-1-PBS cible SMAD1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMAD1 Protéine recombinante Ag0701
• Nom complet: SMAD family member 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC001878
• Symbole du gène: SMAD1
• Identification du gène (NCBI): 4086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

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