Anticorps Polyclonal de lapin anti-SMAD2

• Phare
• Validé par KD/KO

SMAD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

138

N° de cat : 12570-1-AP

Synonymes

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2, SMAD2/3

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Galerie de données de validation

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  WB analysis using 12570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 12570-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 12570-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12570-1-AP

  IP Result of anti-SMAD2 (IP:12570-1-AP, 3ug; Detection:12570-1-AP 1:1000) with HepG2 cells lysate 3000ug.

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  IHC staining of human endometrial cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12570-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMAD2 antibody 12570-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C2C12, cellules C6, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HT-1080, cellules HUVEC, cellules MCF-7, cellules PC-3, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer de l'endomètre humain, tissu de cancer de l'estomac humain, tissu de côlon de rat, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 133 publications below
• IHC: See 9 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

12570-1-AP cible SMAD2 dans les applications de WB, IP, IHC, IF, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD2 Protéine recombinante Ag3237
• Nom complet: SMAD family member 2
• Masse moléculaire calculée: 467 aa, 52 kDa
• Poids moléculaire observé: 52-70 kDa
• Numéro d’acquisition GenBank: BC014840
• Symbole du gène: SMAD2
• Identification du gène (NCBI): 4087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934). The ubiquitination form of Smad2 is ~70 kDa (PMID: 25998442).

Publications

Species	Application	Title
mouse	WB	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
rat	CoIP	Basic Res Cardiol; The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner; Authors - Yongyi Bai
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao
mouse,rat	IHC,WB	Acta Pharmacol Sin; GPR97 deficiency ameliorates renal interstitial fibrosis in mouse hypertensive nephropathy; Authors - Ji-Chao Wu

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