Anticorps Recombinant de lapin anti-SMAD2

SMAD2 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240950G3

N° de cat : 83841-2-PBS

Synonymes

hMAD 2, hMAD-2, hSMAD2, JV18, JV18 1

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Galerie de données de validation

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  WB analysis using 83841-2-RR (same clone as 83841-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83841-2-RR (SMAD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83841-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00791-1

  Sandwich ELISA standard curve of MP00791-1, Human SMAD2 Recombinant Matched Antibody Pair - PBS only. 83841-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3237. 83841-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00791-1

  Serum of sixteen individual healthy human donors was measured. The human SMAD2 concentration of detected samples was determined to be 2.17 ng/mL with a range of 0.34 - 8.22 ng/mL.

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  Sample test of MP00791-1

  HEK-293T, HeLa, HepG2 and MCF-7 cell lysates were measured. The human SMAD2 concentration of detected samples was determined to be 43.85 ng/mL in HEK-293T cell lysate (based on a 5.5 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 33.98 ng/mL in HeLa cell lysate (based on a 7 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 24.50 ng/mL in HepG2 cell lysate (based on a 4.0 mg/mL extract load). The human SMAD2 concentration of detected samples was determined to be 12.02 ng/mL in MCF-7 cell lysate (based on a 3.1 mg/mL extract load).

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  FC experiment of Jurkat using 83841-2-RR (same clone as 83841-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMAD2 Recombinant antibody (83841-2-RR, Clone:240950G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83841-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00791-1

  Cytometric bead array standard curve of MP00791-1, SMAD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83841-2-PBS. Detection antibody: 83841-3-PBS. Standard: Ag3237. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 83841-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83841-2-RR against Human SMAD2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83841-2-PBS cible SMAD2 dans les applications de WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMAD2 Protéine recombinante Ag3237
• Nom complet: SMAD family member 2
• Masse moléculaire calculée: 467 aa, 52 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC014840
• Symbole du gène: SMAD2
• Identification du gène (NCBI): 4087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)

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