Anticorps Recombinant de lapin anti-SMAD2

SMAD2 Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240950G3

N° de cat : 83841-2-RR

Synonymes

hMAD 2, hMAD-2, hSMAD2, JV18, JV18 1

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Galerie de données de validation

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  WB analysis using 83841-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83841-2-RR (SMAD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83841-2-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMAD2 Recombinant antibody (83841-2-RR, Clone:240950G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83841-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83841-2-RR against Human SMAD2 were performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HT-1080, cellules PC-3
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83841-2-RR cible SMAD2 dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMAD2 Protéine recombinante Ag3237
• Nom complet: SMAD family member 2
• Masse moléculaire calculée: 467 aa, 52 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC014840
• Symbole du gène: SMAD2
• Identification du gène (NCBI): 4087
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)

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