Anticorps Polyclonal de lapin anti-SMAD3

SMAD3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30130-1-PBS

Synonymes

hMAD 3, hMAD-3, hSMAD3, HSPC193, HsT17436

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Galerie de données de validation

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  WB analysis using 30130-1-AP (same clone as 30130-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30130-1-AP (SMAD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 30130-1-AP (same clone as 30130-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 30130-1-AP (SMAD3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30130-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30130-1-PBS cible SMAD3 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD3 Protéine recombinante Ag32595
• Nom complet: SMAD family member 3
• Masse moléculaire calculée: 48kd
• Poids moléculaire observé: 48-55 kDa
• Numéro d’acquisition GenBank: NM_005902
• Symbole du gène: SMAD3
• Identification du gène (NCBI): 4088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

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