Anticorps Monoclonal anti-SMAD3

SMAD3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(121)

CloneNo.

1F10B2

Avis (1)

N° de cat : 66516-1-Ig

Synonymes

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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Galerie de données de validation

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  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 66516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 66516-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules 4T1, cellules A431, cellules HEK-293, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 113 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below

Informations sur le produit

66516-1-Ig cible SMAD3 dans les applications de WB, IHC, IF, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, Lapin, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 3
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_005902
• Symbole du gène: SMAD3
• Identification du gène (NCBI): 4088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
human	WB	Cancer Lett; Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma; Authors - Qianyu Shi
human,mouse	WB	Cell Chem Biol; Substrate-dependent interaction of SPOP and RACK1 aggravates cardiac fibrosis following myocardial infarction; Authors - Wanqi Yang
mouse	WB,IF	Cell Mol Life Sci; Macrophage Dectin-1 mediates Ang II renal injury through neutrophil migration and TGF-β1 secretion; Authors - Shiju Ye
human	CoIP	Diabetes; Overexpressed Poldip2 incurs retinal fibrosis via the TGFβ1/SMAD3 signaling pathway in diabetic retinopathy; Authors - Zhiyu Ji

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Juliette (Verified Customer) (11-25-2024)

Good

• Applications: Immunofluorescence
• Cell Tissue Type: Human

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