Anticorps Monoclonal anti-SMAD3

SMAD3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F10B2

N° de cat : 66516-1-PBS

Synonymes

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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Galerie de données de validation

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  WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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  WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66516-1-PBS cible SMAD3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 3
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: NM_005902
• Symbole du gène: SMAD3
• Identification du gène (NCBI): 4088
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

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