Anticorps Polyclonal de lapin anti-SMAD4

SMAD4 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 51069-2-AP

Synonymes

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Galerie de données de validation

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  WB analysis using 51069-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51069-2-AP (SMAD4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 cells using 51069-2-AP

  WB result of SMAD4 antibody (51069-1-AP, 1:1000) with sh-coontrol and sh-SMAD4 transfected HepG2 cells.

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  IHC staining of human tonsillitis using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 51069-2-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 51069-2-AP (SMAD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51069-2-AP (SMAD4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 51069-2-AP (SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51069-2-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 51069-2-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 51069-2-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 51069-2-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 51069-2-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 51069-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (51069-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 51069-2-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human SMAD4 (51069-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules A549, cellules COLO 320, cellules HEK-293, cellules HepG2, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu cardiaque humain, tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

51069-2-AP cible SMAD4 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 4
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC002379
• Symbole du gène: SMAD4
• Identification du gène (NCBI): 4089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human SMAD4.

Publications

Species	Application	Title
rat,mouse	IF	Glia; αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.; Authors - Kathleen K Catignas
human	WB	Chemosphere; PFOS-induced placental cell growth inhibition is partially mediated by lncRNA H19 through interacting with miR-19a and miR-19b.; Authors - Jing Li
mouse	WB,IF	Biochim Biophys Acta Mol Basis Dis; Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the Wnt and TGF-β pathways.; Authors - Reiko Iida
mouse	WB,IF	Physiol Res; Fluid Shear Stress-Induced Down-Regulation of miR-146a-5p Inhibits Osteoblast Apoptosis via Targeting SMAD4; Authors - X Liu