Anticorps Monoclonal anti-SMAD4

SMAD4 Monoclonal Antibody for ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

3H5H1

N° de cat : 60182-1-Ig

Synonymes

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Galerie de données de validation

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  IHC staining of human breast cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 60182-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 60182-1-Ig(SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

60182-1-Ig cible SMAD4 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMAD4 Protéine recombinante Ag0299
• Nom complet: SMAD family member 4
• Masse moléculaire calculée: 60 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC002379
• Symbole du gène: SMAD4
• Identification du gène (NCBI): 4089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a mouse monoclonal antibody raised against an internal region of human SMAD4.

Publications

Species	Application	Title
	IF	Br J Pharmacol; Dual targeting chimeric antigen receptor cells enhance antitumour activity by overcoming T cell exhaustion in pancreatic cancer; Authors - Sun Ruixin
human	WB	Photochem Photobiol Sci; Downregulation of SMAD4 protects HaCaT cells against UVB-induced damage and oxidative stress through the activation of EMT; Authors - Xiangzhi Li

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