Anticorps Monoclonal anti-SMAD5

SMAD5 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

CloneNo.

1A6A4

N° de cat : 67052-1-Ig

Synonymes

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

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Galerie de données de validation

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  WB analysis using 67052-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67052-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Caco-2, cellules HSC-T6, cellules HT-29, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

67052-1-Ig cible SMAD5 dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMAD5 Protéine recombinante Ag28699
• Nom complet: SMAD family member 5
• Masse moléculaire calculée: 465 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC009682
• Symbole du gène: SMAD5
• Identification du gène (NCBI): 4090
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.

Publications

Species	Application	Title
mouse	WB	J Cell Physiol; HIF-1α plays an essential role in BMP9-mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK1.; Authors - Muhammad Subhan Amir
human	WB	Mol Cell Endocrinol; miR-122-5p targets GREM2 to protect against glucocorticoid-induced endothelial damage through the BMP signaling pathway.; Authors - Xianzhe Huang
mouse	WB	FEBS Lett; EGR1 plays an important role in BMP9-mediated osteoblast differentiation by promoting SMAD1/5 phosphorylation.; Authors - Norika Chiba
human	WB	Appl Bionics Biomech; Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway.; Authors - Song Yang
mouse		J Cell Biochem; TLR4/7-mediated host-defense responses of gingival epithelial cells; Authors - Norika Chiba
human,mouse	WB,CoIP	Bone; UBE2C orchestrates bone formation through stabilization of SMAD1/5; Authors - Hui Zhang

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