Anticorps Polyclonal de lapin anti-SMAD6

SMAD6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 18342-1-AP

Synonymes

hSMAD6, MADH6, Mothers against DPP homolog 6, SMAD family member 6

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Galerie de données de validation

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  WB analysis using 18342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18342-1-AP (SMAD6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18342-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18342-1-AP (SMAD6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

18342-1-AP cible SMAD6 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD6 Protéine recombinante Ag13128
• Nom complet: SMAD family member 6
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC012986
• Symbole du gène: SMAD6
• Identification du gène (NCBI): 4091
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD6 is an intracellular signal transduction protein that belongs to the SMAD protein family. It acts as a negative regulator in the transforming growth factor-beta (TGF-β) signaling pathway, binding to TGF-β receptors and inhibiting the phosphorylation of SMAD2/SMAD3, thereby blocking the transmission of TGF-β signals. Additionally, SMAD6 is involved in the regulation of the bone morphogenetic protein (BMP) signaling pathway, affecting cell proliferation, differentiation, and apoptosis. During development, SMAD6 is crucial for the formation of bones and cartilage. Its abnormal expression is associated with various diseases, including skeletal developmental abnormalities and certain types of cancer.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; HNF1B inhibits cell proliferation via repression of SMAD6 expression in prostate cancer.; Authors - Wei Lu

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