Anticorps Polyclonal de lapin anti-SMAD7

• Phare
• Validé par KD/KO

SMAD7 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(109)

N° de cat : 25840-1-AP

Synonymes

CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7

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Galerie de données de validation

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  WB analysis of HepG2 using 25840-1-AP

  WB result of SMAD7 antibody (25840-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD7 transfected HepG2 cells.

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  WB analysis using 25840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25840-1-AP (SMAD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 25840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25840-1-AP (SMAD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 25840-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 25840-1-AP (SMAD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules NIH/3T3, cellules SH-SY5Y, cellules SW480
• Résultats positifs en IHC: tissu hépatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 103 publications below
• IHC: See 9 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

25840-1-AP cible SMAD7 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD7 Protéine recombinante Ag13688
• Nom complet: SMAD family member 7
• Masse moléculaire calculée: 426 aa, 46 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC074819
• Symbole du gène: SMAD7
• Identification du gène (NCBI): 4092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; MicroRNA-181c promotes Th17 cell differentiation and mediates experimental autoimmune encephalomyelitis.; Authors - Zimu Zhang
human	WB	Nat Cell Biol; An ARF6-Exportin-5 axis delivers pre-miRNA cargo to tumour microvesicles.; Authors - James W Clancy
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
mouse	WB	Hypertension; Novel Role for the Immunoproteasome Subunit PSMB10 in Angiotensin II-Induced Atrial Fibrillation in Mice.; Authors - Jing Li
human	WB	Theranostics; Local administration of liposomal-based Srpx2 gene therapy reverses pulmonary fibrosis by blockading fibroblast-to-myofibroblast transition.; Authors - Qi Wang

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