Anticorps Recombinant de lapin anti-SMAD7

SMAD7 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251688E3

N° de cat : 80964-5-PBS

Synonymes

CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7

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Galerie de données de validation

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  WB analysis using 80964-5-RR (same clone as 80964-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80964-5-RR (SMAD7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80964-5-PBS in a different storage buffer formulation.

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Informations sur le produit

80964-5-PBS cible SMAD7 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMAD7 Protéine recombinante Ag13688
• Nom complet: SMAD family member 7
• Masse moléculaire calculée: 426 aa, 46 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC074819
• Symbole du gène: SMAD7
• Identification du gène (NCBI): 4092
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

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