Anticorps Polyclonal de lapin anti-SMAD9

SMAD9 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16397-1-PBS

Synonymes

MAD homolog 9, MADH6, MADH9, Mothers against DPP homolog 9, SMAD 9

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Galerie de données de validation

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  WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16397-1-AP (same clone as 16397-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16397-1-AP (same clone as 16397-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 16397-1-AP (same clone as 16397-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16397-1-AP (same clone as 16397-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 16397-1-AP (same clone as 16397-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 16397-1-AP (same clone as 16397-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16397-1-AP (same clone as 16397-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16397-1-AP (SMAD9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16397-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16397-1-PBS cible SMAD9 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMAD9 Protéine recombinante Ag9490
• Nom complet: SMAD family member 9
• Masse moléculaire calculée: 430 aa, 49 kDa
• Poids moléculaire observé: 49-55 kDa
• Numéro d’acquisition GenBank: BC011559
• Symbole du gène: SMAD9
• Identification du gène (NCBI): 4093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD9 (also known as MADH9 or MAD homolog 9) is a key signaling molecule in the transforming growth factor-β (TGF-β) superfamily signaling pathway and belongs to the SMAD1/5/9 subgroup of receptor-regulated SMADs (R-SMADs). It is primarily activated and phosphorylated by BMP (bone morphogenetic protein) type I receptors. Once activated, SMAD9 forms a complex with the common SMAD4 (Co-SMAD), translocates into the nucleus, and acts as a transcription factor to regulate the expression of specific target genes. This process is involved in modulating a variety of critical biological processes, including cell proliferation, differentiation, apoptosis, and embryonic development. Mutations or abnormal expression of SMAD9 are associated with several diseases, particularly in pulmonary arterial hypertension, certain cancers, and vascular diseases, where it plays a significant role.

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