Anticorps Polyclonal de lapin anti-SMARCA4/BRG1

• Phare
• Validé par KD/KO

SMARCA4/BRG1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 20650-1-AP

Synonymes

SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190

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Galerie de données de validation

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  WB analysis of HeLa using 20650-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20650-1-AP

  WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

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  WB analysis of K-562 using 20650-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20650-1-AP cible SMARCA4/BRG1 dans les applications de WB, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Masse moléculaire calculée: 185 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: NM_001128844
• Symbole du gène: SMARCA4
• Identification du gène (NCBI): 6597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.

Publications

Species	Application	Title
mouse	WB,IF,ChIP	Acta Pharmacol Sin; Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction; Authors - Jing Li
human	WB	J Cell Mol Med; Tyr1497 in the BRG1 Bromodomain of the SWI/SNF Complex is Critical for the Binding and Function of a Selective BRG1 Inhibitor; Authors - Yinan Wang

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