Anticorps Recombinant de lapin anti-SMARCA4/BRG1

SMARCA4/BRG1 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

240172F7

N° de cat : 83310-7-RR

Synonymes

SMARCA4, 240172F7, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190

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Galerie de données de validation

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  WB analysis using 83310-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 83310-7-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 83310-7-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-7-RR, Clone:240172F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83310-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83310-7-RR against Human SMARCA4 were performed. The affinity constant is 1.73 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C6, cellules HEK-293T, cellules HepG2, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du côlon humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83310-7-RR cible SMARCA4/BRG1 dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMARCA4/BRG1 Protéine recombinante Ag16256
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Masse moléculaire calculée: 1647 aa, 185 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC150298
• Symbole du gène: SMARCA4
• Identification du gène (NCBI): 6597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

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