Anticorps Polyclonal de lapin anti-SMARCA4/BRG1

• Phare
• Validé par KD/KO

SMARCA4/BRG1 Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20650-1-PBS

Synonymes

SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190

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Galerie de données de validation

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  WB analysis of HeLa using 20650-1-AP (same clone as 20650-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 20650-1-AP (same clone as 20650-1-PBS)

  WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 20650-1-AP (same clone as 20650-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20650-1-PBS cible SMARCA4/BRG1 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Masse moléculaire calculée: 185 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: NM_001128844
• Symbole du gène: SMARCA4
• Identification du gène (NCBI): 6597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.

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