Anticorps Monoclonal anti-SMARCA4/BRG1

• Phare
• Validé par KD/KO

SMARCA4/BRG1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

CloneNo.

2E6B6

N° de cat : 66561-1-PBS

Synonymes

SMARCA4, 2E6B6, ATP dependent helicase SMARCA4, ATP-dependent helicase SMARCA4, BAF190

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Galerie de données de validation

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  WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  WB result of SMARCA4/BRG1 antibody (66561-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

  IP result of anti-SMARCA4/BRG1 (IP:66561-1-Ig, 5ug; Detection:66561-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66561-1-PBS cible SMARCA4/BRG1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMARCA4/BRG1 Protéine recombinante Ag16256
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Masse moléculaire calculée: 1647 aa, 185 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC150298
• Symbole du gène: SMARCA4
• Identification du gène (NCBI): 6597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

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