Anticorps Recombinant de lapin anti-SMARCC1

SMARCC1 Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250455A1

N° de cat : 86015-1-PBS

Synonymes

BAF155, BRG1-associated factor 155, CRACC1, Rsc8, SRG3

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Galerie de données de validation

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  WB analysis using 86015-1-RR (same clone as 86015-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86015-1-RR (SMARCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

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  Sample test of MP02222-1

  The mean SMARCC1 concentration was determined to be 130.2 ng/mL in Jurkat cell extract based on a 2.2 mg/mL extract load.

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  IF Staining of Jurkat using 86015-1-RR (same clone as 86015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMARCC1 antibody (86015-1-RR, Clone: 250455A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86015-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02222-1

  Cytometric bead array standard curve of MP02222-1, SMARCC1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86015-1-PBS. Detection antibody: 86015-2-PBS. Standard: Ag12109. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 86015-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86015-1-RR against Human SMARCC1 were performed. The affinity constant is 0.393 nM.

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Informations sur le produit

86015-1-PBS cible SMARCC1 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: SMARCC1 Protéine recombinante Ag12109
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1
• Masse moléculaire calculée: 1105 aa, 123 kDa
• Poids moléculaire observé: 155-160 kDa
• Numéro d’acquisition GenBank: BC050564
• Symbole du gène: SMARCC1
• Identification du gène (NCBI): 6599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)

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