Anticorps Polyclonal de lapin anti-SMARCD2

SMARCD2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11156-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of RAW264.7 using 11156-1-AP (same clone as 11156-1-PBS)

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 11156-1-AP (same clone as 11156-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11156-1-AP (SMARCD2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11156-1-AP (SMARCD2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 11156-1-AP (same clone as 11156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 11156-1-AP (SMARCD2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11156-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11156-1-PBS cible SMARCD2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMARCD2 Protéine recombinante Ag1630
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 2
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC018953
• Symbole du gène: SMARCD2
• Identification du gène (NCBI): 6603
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The SWI/SNF chromatin remodelling complexes are important regulators of transcription; they consist of large multisubunit assemblies containing either Brm or Brg1 as the catalytic ATPase subunit and a variable subset of approximately 10 Brg/Brm-associated factors (BAF) [PMID:20148946]. SMARCD2 is one of BAF60 proteins, which are found in most complexes, is thought to bridge interactions between transcription factors and SWI/SNF complexes. [PMID:9427560]

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