Anticorps Monoclonal anti-SMARCE1

SMARCE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F3D6

N° de cat : 66182-1-PBS

Synonymes

BAF57, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1, BRG1-associated factor 57, BRG1 associated factor 57, 1F3D6

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Galerie de données de validation

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  WB analysis of Jurkat using 66182-1-Ig (same clone as 66182-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

  Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SMARCE1 antibody (66182-1-Ig, Clone: 1F3D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66182-1-PBS cible SMARCE1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMARCE1 Protéine recombinante Ag1161
• Nom complet: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007082
• Symbole du gène: SMARCE1
• Identification du gène (NCBI): 6605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.

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