Anticorps Monoclonal anti-SMC1A

SMC1A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

1A5D2

N° de cat : 66987-1-PBS

Synonymes

CDLS2, DXS423E, KIAA0178, Sb1.8, SMC 1 alpha

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Galerie de données de validation

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  WB analysis using 66987-1-Ig (same clone as 66987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66987-1-Ig (SMC1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66987-1-Ig (same clone as 66987-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMC1A Monoclonal antibody (66987-1-Ig, Clone:1A5D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66987-1-PBS cible SMC1A dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMC1A Protéine recombinante Ag17257
• Nom complet: structural maintenance of chromosomes 1A
• Masse moléculaire calculée: 1233 aa, 143 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC112127
• Symbole du gène: SMC1A
• Identification du gène (NCBI): 8243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

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