Anticorps Polyclonal de lapin anti-SMC2

• Phare
• Validé par KD/KO

SMC2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30707-1-PBS

Synonymes

CAP E, CAPE, hCAP E, SMC 2, SMC protein 2

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Galerie de données de validation

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  WB analysis using 30707-1-AP (same clone as 30707-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30707-1-AP (SMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30707-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 30707-1-AP (same clone as 30707-1-PBS)

  WB result of SMC2 antibody (30707-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMC2 transfected HeLa cells. This data was developed using the same antibody clone with 30707-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30707-1-AP (same clone as 30707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30707-1-AP (SMC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30707-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 30707-1-AP (same clone as 30707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30707-1-AP (SMC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30707-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 30707-1-AP (same clone as 30707-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC2 antibody (30707-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 30707-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30707-1-PBS cible SMC2 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC2 Protéine recombinante Ag33471
• Nom complet: structural maintenance of chromosomes 2
• Masse moléculaire calculée: 136 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: NM_001042550.1
• Symbole du gène: SMC2
• Identification du gène (NCBI): 10592
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).

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