Anticorps Polyclonal de lapin anti-SMC3

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 14185-1-AP

Synonymes

Chromosome-associated polypeptide, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Bamacan

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Galerie de données de validation

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  WB analysis of HeLa using 14185-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14185-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14185-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14185-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human stomach using 14185-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14185-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14185-1-AP cible SMC3 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC3 Protéine recombinante Ag5440
• Nom complet: structural maintenance of chromosomes 3
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC047324
• Symbole du gène: SMC3
• Identification du gène (NCBI): 9126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	CoIP	Int J Mol Sci; APEX2-Mediated Proximity Labeling Resolves the DDIT4-Interacting Proteome.; Authors - Marianna Naki
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali
mouse	WB	Cell Mol Life Sci; RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells; Authors - Wanzhi Cai
human	WB	Heliyon; ZNF521 promotes acute myeloid leukemogenesis by suppressing the expression and acetylation of SMC3; Authors - Rong Qin
human	WB	Curr Eye Res; Cohesin Complex Interacting with Promoters of MMP Genes for in Pterygium Occurrence; Authors - Shichao Han ; KD Validated
	IF	Proc Natl Acad Sci U S A; Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta; Authors - Talia Hatkevich

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