Anticorps Polyclonal de lapin anti-SMC3

SMC3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 14185-1-PBS

Synonymes

Chromosome-associated polypeptide, CDLS3, BMH, Basement membrane-associated chondroitin proteoglycan, Bamacan

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Galerie de données de validation

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  WB analysis of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 14185-1-AP (same clone as 14185-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14185-1-AP (same clone as 14185-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 14185-1-AP (same clone as 14185-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 14185-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14185-1-AP (SMC3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human stomach using 14185-1-AP (same clone as 14185-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14185-1-AP (SMC3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 14185-1-AP (same clone as 14185-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14185-1-AP (SMC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14185-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14185-1-PBS cible SMC3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC3 Protéine recombinante Ag5440
• Nom complet: structural maintenance of chromosomes 3
• Masse moléculaire calculée: 142 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC047324
• Symbole du gène: SMC3
• Identification du gène (NCBI): 9126
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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