Anticorps Polyclonal de lapin anti-SMC4

• Phare
• Validé par KD/KO

SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24758-1-PBS

Synonymes

CAP C, CAPC, Chromosome-associated polypeptide C, hCAP C, hCAPC

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Galerie de données de validation

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  WB analysis using 24758-1-AP (same clone as 24758-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24758-1-AP (SMC4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 24758-1-AP (SMC4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 24758-1-AP (same clone as 24758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24758-1-AP (SMC4 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 24758-1-AP (same clone as 24758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMC4 antibody (24758-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24758-1-PBS in a different storage buffer formulation.

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Informations sur le produit

24758-1-PBS cible SMC4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC4 Protéine recombinante Ag19156
• Nom complet: structural maintenance of chromosomes 4
• Masse moléculaire calculée: 1288 aa, 147 kDa
• Poids moléculaire observé: 147-150 kDa
• Numéro d’acquisition GenBank: BC106033
• Symbole du gène: SMC4
• Identification du gène (NCBI): 10051
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

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