Anticorps Polyclonal de lapin anti-SMC5

• Phare
• Validé par KD/KO

SMC5 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 14178-1-AP

Synonymes

SMC5L1, SMC-5, SMC protein 5, SMC 5, KIAA0594

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Galerie de données de validation

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  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14178-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14178-1-AP (SMC5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14178-1-AP cible SMC5 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMC5 Protéine recombinante Ag5385
• Nom complet: structural maintenance of chromosomes 5
• Masse moléculaire calculée: 1101 aa, 129 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC038225
• Symbole du gène: SMC5
• Identification du gène (NCBI): 23137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	mSystems; Adenovirus Remodeling of the Host Proteome and Host Factors Associated with Viral Genomes; Authors - Joseph M. Dybas ; KD Validated
human	IF	J Virol; PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner.; Authors - Wei Xu
human	WB	Cell Rep; Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.; Authors - Stephanie Pei Tung Yiu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-30-2022)

The detection of SMC5 by WB is fair with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HepG2 cell

SMC5 Antibody Western Blot validation (1/1000 dilution) in HepG2 cell (Cat no:14178-1-AP)

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