Anticorps Monoclonal anti-SMCR7L

SMCR7L Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3B3G3

N° de cat : 67808-1-PBS

Synonymes

dJ1104E15.3, FLJ20232, HSU79252, MID51, MIEF1, SMCR7L

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Galerie de données de validation

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  WB analysis using 67808-1-Ig (same clone as 67808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67808-1-Ig (SMCR7L/MID51 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67808-1-Ig (same clone as 67808-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SMCR7L/MID51 antibody (67808-1-Ig, Clone: 3B3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67808-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67808-1-PBS cible SMCR7L dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMCR7L Protéine recombinante Ag13775
• Nom complet: Smith-Magenis syndrome chromosome region, candidate 7-like
• Masse moléculaire calculée: 463 aa, 51 kDa
• Poids moléculaire observé: 48-51 kDa
• Numéro d’acquisition GenBank: BC002587
• Symbole du gène: SMCR7L
• Identification du gène (NCBI): 54471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Human SMCR7L gene encodes, MID51, the mitochondrial dynamic protein of 51 kDa (also called mitochondrial elongation factor 1, MIEF1). MID51 is a single-pass membrane protein anchored to the mitochondrial outer membrane and regulates mitochondrial morphology. Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Elevated MID51 levels induce extensive mitochondrial fusion, whereas depletion of MID51 causes mitochondrial fragmentation. MID51 interacts with and recruits Drp1 to mitochondria, suggesting a critical role of MID51 in regulation of mitochondrial fusion-fission machinery in vertebrates.

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