Anticorps Polyclonal de lapin anti-SMEK1

SMEK1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26153-1-PBS

Synonymes

KIAA2010, PP4R3A, PPP4R3A

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Galerie de données de validation

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  WB analysis using 26153-1-AP (same clone as 26153-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26153-1-AP (SMEK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 26153-1-AP (same clone as 26153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26153-1-AP (SMEK1 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 26153-1-AP (same clone as 26153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMEK1 antibody (26153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 26153-1-PBS in a different storage buffer formulation.

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Informations sur le produit

26153-1-PBS cible SMEK1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SMEK1 Protéine recombinante Ag5070
• Nom complet: SMEK homolog 1, suppressor of mek1 (Dictyostelium)
• Masse moléculaire calculée: 833 aa, 95 kDa
• Poids moléculaire observé: 95-100 kDa
• Numéro d’acquisition GenBank: BC038932
• Symbole du gène: SMEK1
• Identification du gène (NCBI): 55671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPP4R3A also known as SMEK1, is the regulatory subunit of serine/threonine-protein phosphatase 4. SMEK1 regulates the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair(PMID: 18614045).

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