Anticorps Monoclonal anti-SMG6

SMG6 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D2G2

N° de cat : 68109-1-PBS

Synonymes

C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A

---

Galerie de données de validation

• 

  WB analysis using 68109-1-Ig (same clone as 68109-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68109-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 68109-1-Ig (same clone as 68109-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68109-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68109-1-PBS cible SMG6 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMG6 Protéine recombinante Ag30182
• Nom complet: Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
• Masse moléculaire calculée: 160 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_017575
• Symbole du gène: SMG6
• Identification du gène (NCBI): 23293
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMG6, also known as C17orf31, EST1A, and hEST1A, is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PMID: 19179534). It promotes in vitro the ability of TERT to elongate telomeres (PMID: 12676087, 12699629). SMG6 also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD (PMID: 19060897, 20930030). Alternate splicing results in multiple transcript variants (PMID: 12699629, 14636577).

{{ptg:RelatedPrimaryAntibodies}}