Anticorps Monoclonal anti-SMMHC

SMMHC Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

5C6D3

N° de cat : 60222-1-PBS

Synonymes

MYH11, 5C6D3, KIAA0866, Myosin 11, Myosin heavy chain 11

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Galerie de données de validation

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  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 60222-1-Ig (same clone as 60222-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60222-1-PBS cible SMMHC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: SMMHC Protéine recombinante Ag13397
• Nom complet: myosin, heavy chain 11, smooth muscle
• Masse moléculaire calculée: 1938 aa, 224 kDa
• Poids moléculaire observé: 220 kDa
• Numéro d’acquisition GenBank: BC101677
• Symbole du gène: SMMHC/MYH11
• Identification du gène (NCBI): 4629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.

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